

Dr.
Stroot and his students have exclusive use of approximately 950 square feet of contiguous
research space (one lab for microbiology, one lab for molecular biology, and one
room designated for microscopic analysis). Dr. Stroot and his students utilize 350
square feet of office space that is adjacent to the research labs. Offices are equipped
with computers and common laser printer that is available to the research group.
One computer is dedicated to the Kodak EDAS 290 Gel Imaging system.
The
microbiology lab is equipped for aerobic and anaerobic microbiology, pathogen work,
and bioreactors. For anaerobic digester work, the lab contains two upright temperature
control units (Fisher IsoTemp incubators), and a constant temperature shaker platform
for preparation of anaerobic microbial cultures (Amerex Gyromax 727), and anaerobic
cultures can be prepared in an anaerobic chamber (Coy Type B). An autoclave (Amerex
AT-HV-85) is available for culture and equipment sterilization. The anaerobic bioreactors
include two custom-designed biological waste treatment reactors including gas collection,
while Balch bottles and tubes are available for small-scale work. Two Fisher IsoTemp
upright incubators control the working temperature of the anaerobic bioreactors.
Solids analysis of bioreactor samples is possible through use of a Fisher drying
oven and Thermolyne muffle furnace.
The molecular biology lab is equipped for sample storage and various methods of
interrogation. Samples and pure cultures are stored in an ultra-low temperature
freezer (Revco). A spectrophotometer with quartz cuvettes (Genosys model 10 UV/Vis)
measures the concentration of nucleic acid. DNA and RNA can be extracted with a
large volume centrifugation (Eppendorf model 5810R), cell disrupter (BioSpec mini-bead
beater), and water baths with recirculating heaters (Fisher IsoTemp models 215 and
220). PCR is possible with a BioRad MyCycler Gradient Thermocycler. DNA and RNA
abundance and size is determined with equipment for agarose gel electrophoresis
(BioRad) and a gel imaging system (Kodak EDAS 290).
The microscopy lab is equipped with an upright epifluorescence microscope (Leitz
Diaplan) with a digital camera (SPOT Flex by Diagnostic Instruments) and computer
(Dell).



